Project Overview

Dr. Donna Peters of the Department of Pathology here at UW-Madison aims to test the effects of various drugs on the movement of fluid across a layer of human eye cells. Currently, the eye cells are supported on a silicon membrane that simulates the flexibility of the tissue in vivo. The membrane is placed in an Ussing perfusion chamber that measures the effects of pressure on the cell layer. This experimental system must be redesigned to allow greater control of the experimental conditions. Specifically, the chamber must allow for control of the movement of fluid, easy replacement of cell culture plates, and measurement of fluid pressure with computer-controlled transducers. In addition, a porous elastic membrane that permits fluid flow will replace the silicon membrane of the current system. A successful design will be used to screen for potential treatments of glaucoma.

Files

• Midsemester Presentation (October 21, 2007)
• Product Design Specifications (Revised 10-24-07) (October 24, 2007)
• Preliminary Design Report (December 5, 2007)
• Final Design Report (December 13, 2007)
• Final Poster Presentation (December 14, 2007)

Contact Information

Team Members

• Holly Liske - Team Leader
• Laura Piechura - Communicator
• Kellen Sheedy - BSAC
• Joseph Labuz - BWIG

Advisor and Client

• Prof. William Murphy - Advisor
• Dr. Donna Peters - Client