CRISPRi screening in cancer spheroids to investigate factors in genome stability
Develop a cell culture method compatible with a 3D environment and CRISPRi screening in order to identify sources of DNA mutations in the tumor environment through γH2AX expression.
Project Overview
Although previous CRISPRi screening in 2D monolayers has provided useful knowledge on cancer drivers and therapeutic susceptibilities, it lacks an element of biological relevance to an in vivo environment. Therefore, our team was tasked with developing a cell culture method that is compatible with a 3D environment and CRISPRi screening in order to identify sources of DNA mutations in the tumor environment. On a high scale, the team must select a viable cell line for the screen, create and optimize a spheroid formation protocol, and develop a protocol to stain for γH2AX: a histone variant that acts as a sensitive marker for DNA damage.
Project Status
The team is finished the poster presentation, final report, and notebook.
Team Pictures


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Files
- Final Notebook (May 7, 2025)
- Final Report (May 6, 2025)
- Final Poster Presentation (May 5, 2025)
- PDS (May 5, 2025)
- Executive Summary (April 18, 2025)
- Preliminary Report (March 4, 2025)
- Preliminary Presentation (February 7, 2025)
Progress Reports
- Week 13 (April 23, 2025)
- Week 12 (April 16, 2025)
- Week 11 (April 9, 2025)
- Week 10 (April 2, 2025)
- Week 9 (March 26, 2025)
- Week 8 (March 19, 2025)
- Week 7 (March 13, 2025)
- Week 6 (March 6, 2025)
- Week 5 (February 27, 2025)
- Week 4 (February 20, 2025)
- Week 3 (February 13, 2025)
- Week 2 (February 6, 2025)
- Week 1 (January 30, 2025)
Contact Information
Team Members
- Althys Cao - Team Leader
- Ana Martinez - Communicator
- Emily Rhine - BSAC
- Julia Salita - BWIG
- Jayson O'Halloran - BPAG
Advisor and Client
- Prof. Paul Campagnola - Advisor
- Ms. Carley Schwartz - Client
- Dr. Gaelen Hess - Alternate Contact
Related Projects
- Spring 2025: CRISPRi screening in cancer spheroids to investigate factors in genome stability
- Fall 2024: CRISPRi screening in cancer spheroids to investigate factors in genome stability