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Tissue biopsy dissociation

Project Overview

Biological research often requires the study of individual cells to gain a better understanding of processes within the human body. The client’s research on asthma has focused on the use of tissue biopsy dissociation to obtain individual cells. The research is specifically interested in studying cells before and after an induced asthmatic response. The current device being used for this process, the gentleMACS™ Dissociation Device, did not allow for use of a small tissue sample size. A small tissue sample size, 1-2 mm, was desired to reduce the recovery time and pain of the patient. The team was tasked to create a dissociation device that would successfully dissociate smaller tissue samples and yield viable cells to study. The most important criteria considered in the final design was the ability of the design to perform appropriately and yield at least 50% viable cells. A microfluidic device was used to dissociate this small tissue sample and testing was be conducted to determine the effectiveness of this device. Statistical analysis on the results indicated no difference in cell dissociation when the device was used. Future work proposes methods for increasing cell yield based on the current design.

Team Picture

Team members from left to right: Nate Richman, Cory Van Beek, Jamison Miller, Professor Li, Victoria Trantow, Lauren Ross, Raven Brenneke
Team members from left to right: Nate Richman, Cory Van Beek, Jamison Miller, Professor Li, Victoria Trantow, Lauren Ross, Raven Brenneke

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Contact Information

Team Members

  • Raven Brenneke - Team Leader
  • Jamison Miller - Co-Communicator
  • Nathan Richman - Co-Communicator
  • Lauren Ross - BSAC
  • Victoria Trantow - BWIG
  • Cory Van Beek - BPAG

Advisor and Client

  • Prof. Wan-Ju Li - Advisor
  • Dr. Sameer Mathur - Client

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