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Microfluidic sensing chip: Microfluidic device for long-term maintenance of biosensing cells

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Microfluidic chip capable of housing colonies of biosensing yeast cells long-term for detection of human endocrine disruptors

Project Overview

This project will design a microfluidic device for maintaining yeast cells at constant density and in a single plane (i.e. monolayer). These yeast cells contain sensing proteins that allow them to detect human endocrine disruptors (HEDCs) and express fluorescent proteins in response to the presence of these HEDCs. The device must allow for replenishing these yeast biosensors with appropriate nutrients and delivering samples to be tested for HEDCs to the yeast. Any yeast growth within the device must be handled so that the yeast stays at a constant density and number. Finally, an ideal device would contain multiple compartments for yeast with different HEDCs sensors.

Team Picture

from left to right: Amy, Sam, Margo, Bella, Tony, Ren
from left to right: Amy, Sam, Margo, Bella, Tony, Ren

Contact Information

Team Members

  • Tony Tu - Team Leader
  • Amy Cao - Communicator
  • Margo Amatuzio - Co-BSAC
  • Samuel Neuman - Co-BSAC
  • Bella Boeckenstedt - BWIG
  • Ren Babu - BPAG

Advisor and Client

  • Dr. Melissa Kinney - Advisor
  • Prof. Megan McClean - Client
  • Prof. Bhuvana Krishnaswamy - Alternate Contact

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