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Microscope cell culture incubator

The team is tasked with making a microscope top incubator that can mimic cellular conditions for up to a week at a time.

Project Overview

To support live cell imaging in the BME teaching lab, the client needs a cell culture incubator that fits on the stage of a Nikon TI-U Inverted Fluorescence Microscope. The device must maintain constant environmental conditions (37℃, 100% Humidity, and 5% CO2 concentration) for up to one week. Moreover, the incubator must have an optically clear top and bottom that do not interfere with fluorescence imaging. The device should accommodate different types of cell culture containers including multiwell plates, chamber slides, rectangular dishes, and Petri dishes. The client would like the device to display the environmental conditions and notify the user if conditions deviate from an acceptable range. Commercially available devices satisfy these design requirements, although they are expensive; the client’s budget is $100 per device.

Team Picture

Tim Madigan, Kevin Koesser, Bob Meuler, and Nick Pauly
Tim Madigan, Kevin Koesser, Bob Meuler, and Nick Pauly

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Contact Information

Team Members

  • Nicholas Pauly - Team Leader
  • Timothy Madigan - Communicator
  • Kevin Koesser - BSAC
  • Robert Meuler - BWIG & BPAG

Advisor and Client

  • Prof. Paul Campagnola - Advisor
  • Prof. John Puccinelli - Client

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