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Microscope low-cost motorized stage

Create a precise motorized stage for an inverted fluorescence microscope for under $100 so that more precise and efficient measurements can be done, while making it accessible to those with inadequate funding to purchase expensive alternatives.

Project Overview

The two inverted fluorescent microscopes in the teaching lab of the Engineering Centers Building utilize stages that must be manually moved with two adjustable knobs. This method of adjusting the stage is not precise and does not allow for automated imaging of coordinate based locations. The current adjustment system also does not allow for a reference of the distance traveled which further decreases the precision of the instrument. Solutions for this problem do exist, however, they are costly. Kits and instructions to fix this problem go only as low as approximately $300 dollars. Microscopes with built in motorized adjustment systems can be as expensive as $70,000 new and $19,000 used. To increase the availability and widespread use of this type of solution, we aim to build ours for under $100. Our goal is to improve upon the previous designs, maintain accuracy of the instrument throughout the testing process, and to have a movement resolution of one micrometer (1µm).

Team Picture

Team Members depicted in team photo; Far left: Zhaoyun/Jerry Tang, Middle Left: Nick Symons, Middle: Sawyer Bussey, Middle right: Tyler Haupert, Far right: Julia Salita
Team Members depicted in team photo; Far left: Zhaoyun/Jerry Tang, Middle Left: Nick Symons, Middle: Sawyer Bussey, Middle right: Tyler Haupert, Far right: Julia Salita

Images

Team members Sawyer Bussey (left), Tyler Haupert (center) and Jerry Tang(Right). Fabrication and assembly in the makerspace
Team members Sawyer Bussey (left), Tyler Haupert (center) and Jerry Tang(Right). Fabrication and assembly in the makerspace
Photo of Nick and Julia conducting testing procedures
Photo of Nick and Julia conducting testing procedures
Nick working on software and electrical components for the stepper motors
Nick working on software and electrical components for the stepper motors
One side of the stepper motor housing unit that has yet to be installed onto the sled
One side of the stepper motor housing unit that has yet to be installed onto the sled
A control knob gear that was damaged after an attempt to insert heated threads into the plastic
A control knob gear that was damaged after an attempt to insert heated threads into the plastic
One of the final CAD files for the stepper motor housing unit
One of the final CAD files for the stepper motor housing unit
Early Version of Stepper motor 3D printed gear
Early Version of Stepper motor 3D printed gear
Early version of stepper motor housing unit
Early version of stepper motor housing unit
Fabrication- Mounting the Linear rail to the GMP
Fabrication- Mounting the Linear rail to the GMP
Side view of near final product with tape to secure the control knob
Side view of near final product with tape to secure the control knob

Files

Contact Information

Team Members

  • Tyler Haupert - Team Leader
  • Nicholas Symons - Communicator
  • Julia Salita - BSAC
  • Sawyer Bussey - BWIG
  • Zhaoyun Tang - BPAG

Advisor and Client

  • Prof. Joshua Brockman - Advisor
  • Prof. John Puccinelli - Client

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